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<section id="phasorpy">
<h1>PhasorPy<a class="headerlink" href="#phasorpy" title="Link to this heading">#</a></h1>
<p>PhasorPy is an open-source Python library for the analysis of luminescence
lifetime and hyperspectral images using the phasor approach.</p>
<p>The phasor approach represents time-resolved and spectral signals as
phasor coordinates (normalized Fourier coefficients at harmonic frequencies)
for intuitive visualization and analysis.</p>
<p>PhasorPy enables reproducible phasor-based fluorescence lifetime imaging
(FLIM) and hyperspectral imaging (HSI) workflows in scientific Python.
It provides tools to read microscopy data in many file formats and to
calculate, calibrate, filter, visualize, and interconvert phasor coordinates,
lifetimes, and signals. Phasor coordinates can be exported to standard
formats and analyzed through cursor-based region-of-interest selection,
cluster detection, multi-component unmixing, FRET efficiency and concentration
estimation.</p>
<div class="sphx-glr-thumbnails docutils container">
<div class="sphx-glr-thumbcontainer" tooltip="Demonstrate key features of the PhasorPy library."><img alt="Introduction to PhasorPy" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_introduction_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/phasorpy_introduction"><span>Introduction to PhasorPy</span></a></p><div class="sphx-glr-thumbnail-title">Introduction to PhasorPy</div></div><div class="sphx-glr-thumbcontainer" tooltip="Illustrate the geometric interpretation of lifetimes in the phasor plot."><img alt="Geometric interpretation of lifetimes" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_lifetime_geometry_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/phasorpy_lifetime_geometry"><span>Geometric interpretation of lifetimes</span></a></p><div class="sphx-glr-thumbnail-title">Geometric interpretation of lifetimes</div></div><div class="sphx-glr-thumbcontainer" tooltip="Read and write phasor-related data in various file formats."><img alt="File input/output" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_io_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/api/phasorpy_io"><span>File input/output</span></a></p><div class="sphx-glr-thumbnail-title">File input/output</div></div><div class="sphx-glr-thumbcontainer" tooltip="Calculate and plot phasor coordinates of FRET donor and acceptor channels."><img alt="Förster resonance energy transfer" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_fret_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/api/phasorpy_fret"><span>Förster resonance energy transfer</span></a></p><div class="sphx-glr-thumbnail-title">Förster resonance energy transfer</div></div><div class="sphx-glr-thumbcontainer" tooltip="Explore phasor concepts using interactive educational applications."><img alt="Interactive applications" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_apps_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/misc/phasorpy_apps"><span>Interactive applications</span></a></p><div class="sphx-glr-thumbnail-title">Interactive applications</div></div><div class="sphx-glr-thumbcontainer" tooltip="Spectral unmixing using multi-component analysis in phasor space."><img alt="Spectral unmixing" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_component_fit_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/applications/phasorpy_component_fit"><span>Spectral unmixing</span></a></p><div class="sphx-glr-thumbnail-title">Spectral unmixing</div></div><div class="sphx-glr-thumbcontainer" tooltip="Determine absolute NADH concentration using intensity-calibrated phasor FLIM."><img alt="Absolute NADH concentration" src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_nadh_concentration_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/applications/phasorpy_nadh_concentration"><span>Absolute NADH concentration</span></a></p><div class="sphx-glr-thumbnail-title">Absolute NADH concentration</div></div><div class="sphx-glr-thumbcontainer" tooltip="Browse all PhasorPy tutorials."><img alt="All tutorials..." src="https://www.phasorpy.org/docs/stable/_images/sphx_glr_phasorpy_logo_thumb.png" /><p><a class="reference internal" href="https://www.phasorpy.org/docs/stable/tutorials/"><span>All tutorials...</span></a></p><div class="sphx-glr-thumbnail-title">All tutorials...</div></div></div>
<section id="documentation">
<h2>Documentation<a class="headerlink" href="#documentation" title="Link to this heading">#</a></h2>
<p>The <a class="reference external" href="docs/stable/">PhasorPy documentation</a> thoroughly documents all aspects
of the library, including:</p>
<ul class="simple">
<li><p><a class="reference external" href="docs/stable/phasor_approach/">Phasor approach</a></p></li>
<li><p><a class="reference external" href="docs/stable/tutorials/">Tutorials</a></p></li>
<li><p><a class="reference external" href="docs/stable/api/">API reference</a></p></li>
<li><p><a class="reference external" href="docs/stable/release/">Release notes</a></p></li>
<li><p><a class="reference external" href="docs/stable/contributing/">Contributing guide</a></p></li>
<li><p><a class="reference external" href="docs/stable/acknowledgments/">Acknowledgments</a></p></li>
<li><p><a class="reference external" href="docs/stable/license/">MIT License</a></p></li>
</ul>
<p>Other versions: <a class="reference external" href="docs/dev">latest development</a>, <a class="reference internal" href="docs/"><span class="doc">all</span></a></p>
</section>
<section id="resources">
<h2>Resources<a class="headerlink" href="#resources" title="Link to this heading">#</a></h2>
<ul class="simple">
<li><p><a class="reference external" href="https://github.com/phasorpy/phasorpy">Source code</a> is maintained on GitHub.com.</p></li>
<li><p><a class="reference external" href="https://pypi.org/project/phasorpy/">Releases</a> are published on PyPI.org and <a class="reference external" href="https://anaconda.org/conda-forge/phasorpy">conda-forge</a>.</p></li>
<li><p><a class="reference external" href="https://zenodo.org/communities/phasorpy">Data files</a> are shared on Zenodo.org.</p></li>
<li><p><a class="reference external" href="https://github.com/phasorpy/phasorpy/issues">Ask questions and report issues</a> on the GitHub issue tracker.</p></li>
</ul>
</section>
<section id="news">
<h2>News<a class="headerlink" href="#news" title="Link to this heading">#</a></h2>
<ul class="simple">
<li><p>July 2, 2026: <a class="reference external" href="https://github.com/phasorpy/phasorpy/graphs/contributors">300th commit</a> to the PhasorPy source code.</p></li>
<li><p>June 6, 2026: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.11</a> is released to PyPI.</p></li>
<li><p>May 12, 2026: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.10</a> is released to PyPI.</p></li>
<li><p>Jan 1, 2026: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.9</a> is released to PyPI.</p></li>
<li><p>Oct 10, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.8</a> is released to PyPI.</p></li>
<li><p>Aug 22, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.7</a> is released to PyPI.</p></li>
<li><p>Aug 16, 2025: The new <a class="reference external" href="https://www.phasorpy.org/_static/phasorpy_logo.svg">PhasorPy logo</a> is live.</p></li>
<li><p>June 22, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.6</a> is released to PyPI.</p></li>
<li><p>June 16, 2025: <a class="reference external" href="https://github.com/phasorpy/phasorpy/graphs/contributors">200th commit</a> to the PhasorPy source code.</p></li>
<li><p>Apr 11, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.5</a> is released to PyPI.</p></li>
<li><p>Jan 30, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.4</a> is released to PyPI.</p></li>
<li><p>Jan 25, 2025: The <a class="reference external" href="https://github.com/cgohlke/liffile">liffile library</a> for reading Leica LIF files is released.</p></li>
<li><p>Jan 8, 2025: PhasorPy is available on <a class="reference external" href="https://anaconda.org/conda-forge/phasorpy/">conda-forge</a>.</p></li>
<li><p>Dec 16, 2024: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.3</a> is released to PyPI.</p></li>
<li><p>Nov 30, 2024: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.2</a> is released to PyPI.</p></li>
<li><p>Oct 22, 2024: PhasorPy project update and practical are presented at the <a class="reference external" href="https://www.lfd.uci.edu/workshop/">18th LFD Workshop</a>.</p></li>
<li><p>Sept 30, 2024: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.1</a> is released to PyPI.</p></li>
<li><p>Sept 23, 2024: <a class="reference external" href="https://github.com/phasorpy/phasorpy/graphs/contributors">100th commit</a> to the PhasorPy source code.</p></li>
<li><p>June 12, 2024: PhasorPy is featured at the <a class="reference external" href="https://chanzuckerberg.com/science/programs-resources/open-science/">CZI Open Science 2024</a> meeting.</p></li>
<li><p>Feb 16, 2024: First <a class="reference external" href="https://www.phasorpy.org/docs/stable/tutorials/">tutorial</a> added to the documentation.</p></li>
<li><p>Nov 1, 2023: The <a class="reference external" href="https://github.com/cgohlke/ptufile">ptufile library</a> for reading PicoQuant PTU files is released.</p></li>
<li><p>Oct 26, 2023: The PhasorPy project is introduced at the <a class="reference external" href="https://www.lfd.uci.edu/workshop/2023/">17th LFD Workshop</a>.</p></li>
<li><p>Oct 5, 2023: The PhasorPy <a class="reference external" href="https://zenodo.org/communities/phasorpy">community on Zenodo</a> is used to share data files.</p></li>
<li><p>Sept 1, 2023: <a class="reference external" href="https://github.com/bruno-pannunzio">Bruno Pannunzio</a> joins the PhasorPy project.</p></li>
<li><p>Aug 22, 2023: The library documentation is published at <a class="reference external" href="https://www.phasorpy.org/docs/stable">www.phasorpy.org</a>.</p></li>
<li><p>Aug 21, 2023: First PhasorPy developer meeting, to be held remotely every two weeks.</p></li>
<li><p>July 6, 2023: PhasorPy’s <a class="reference external" href="https://github.com/phasorpy/phasorpy">organization and repository on GitHub</a> are live.</p></li>
<li><p>July 1, 2023: <a class="reference external" href="https://github.com/cgohlke">Christoph Gohlke</a> joins the PhasorPy project.</p></li>
<li><p>Sept 2022: A <a class="reference external" href="https://chanzuckerberg.com/eoss/proposals/phasorpy-a-python-library-for-phasor-analysis-of-flim-and-spectral-imaging/">grant from the Chan Zuckerberg Initiative</a>
is awarded to Leonel Malacrida and Enrico Gratton for the development of the PhasorPy library and community.</p></li>
</ul>
</section>
<section id="events">
<h2>Events<a class="headerlink" href="#events" title="Link to this heading">#</a></h2>
<p>The PhasorPy project is presented at the following events:</p>
<ul class="simple">
<li><p>Bruno Pannunzio.
PhasorPy and napari-phasors: open source tools for FLIM-phasor analysis.
<a class="reference external" href="https://ppbi.pt/wordpress/index.php/f-techniques-worskhop-series-flim-january-13-15-2026/">F-Techniques Worskhop Series: FLIM</a>.
January 13-15, 2026. Portuguese Platform of BioImaging (PPBI), Portugal.</p></li>
<li><p>Bruno Schüty.
PhasorPy hands-on training.
<a class="reference external" href="https://sites.uci.edu/aimlfdworkshop/">LFD/AIM Workshop on Fluorescence Advanced Imaging Research (FLAIR)</a>.
January 12-15, 2026. University of California, Irvine, USA.</p></li>
<li><p>Bruno Pannunzio and Leonel Malacrida.
The PhasorPy library for FLIM and HSI phasor analysis.
<a class="reference external" href="https://pasteur.uy/cursos-y-charlas/6th-workshop-in-advanced-microscopy-and-biophotonics/">6th Workshop in Advanced Microscopy and Biophotonics</a>.
November 23-28, 2025. Institut Pasteur de Montevideo, Uruguay.</p></li>
<li><p>Leonel Malacrida.
PhasorPy library for open-source phasor analysis of FLIM and HSI: present, future and some applications in biological fluorescence.
<a class="reference external" href="https://www.webersymposium.com/">12th International Weber Symposium</a>.
June 15-20, 2025. Genoa, Italy.</p></li>
<li><p>Bruno Pannunzio, Bruno Schuty Teske, Michelle Digman, Christoph Gohlke, and Leonel Malacrida.
PhasorPy: an open-source Python library for the analysis of fluorescence lifetime and hyperspectral images using the phasor approach.
<a class="reference external" href="https://biophysics.cld.bz/BPS2025-Full-Program-Abstracts/125/">69th Annual Meeting of the Biophysical Society</a>.
February 15-19, 2025. Los Angeles, USA.</p></li>
<li><p>Leonel Malacrida.
Introduction to PhasorPy.
Third LFD around the world meeting.
December 9, 2024. Online.</p></li>
<li><p>Leonel Malacrida.
The PhasorPy library for FLIM and HSI phasor analysis.
<a class="reference external" href="https://www.optica.org/events/webinar/2024/11_november/the_phasorpy_library_for_flim_and_hsi_phasor_analysis/">Optica Webinar Series</a>.
November 25, 2024. Online.
<a class="reference external" href="https://www.optica.org/events/webinar/2024/11_november/the_phasorpy_library_for_flim_and_hsi_phasor_analysis/">Recording</a>.</p></li>
<li><p>Bruno Schüty.
Phasor Analysis in fluorescence lifetime imaging and spectral imaging with PhasorPy.
<a class="reference external" href="https://www.globias.org/activities/past-activties/annual-workshop-gothenburg-2024">GloBIAS Bioimage Analysts Workshop</a>.
November 5-8, 2024. Gothenburg, Sweden.
<a class="reference external" href="https://www.youtube.com/watch?v=5qURI2NVkzg/">Recording</a>.</p></li>
<li><p>Bruno Pannunzio.
PhasorPy: an open-source Python library for the analysis of fluorescence lifetime and hyperspectral images using the phasor approach.
<a class="reference external" href="https://www.i2kconference.org/workshops">Virtual I2K 2024: Online tutorials on image analysis</a>.
October 29, 2024. Online.
<a class="reference external" href="https://www.youtube.com/watch?v=VGKGF8Zj3tY">Recording</a>.</p></li>
<li><p>Leonel Malacrida.
Unlocking the phasor approach for FLIM and HSI: Insights from PhasorPy, a comprehensive Python library for phasor plot analysis.
<a class="reference external" href="https://www.eurobioimaging.eu/events/phasor-plots-for-hyperspectral-imaging-and-flim/">Euro-Bioimaging Virtual Pub</a>.
October 25, 2024. Online.
<a class="reference external" href="https://www.youtube.com/watch?v=cI7WydgIG00">Recording</a>.</p></li>
<li><p>Bruno Schüty.
PhasorPy hands-on training.
<a class="reference external" href="https://www.lfd.uci.edu/workshop/">18th LFD Workshop in Advanced Fluorescence Imaging and Dynamics</a>.
October 21-24, 2024.
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA.</p></li>
<li><p>Christoph Gohlke.
Introduction to PhasorPy.
<a class="reference external" href="https://www.lfd.uci.edu/workshop/">18th LFD Workshop in Advanced Fluorescence Imaging and Dynamics</a>.
October 21-24, 2024.
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA.</p></li>
<li><p>Bruno Pannunzio, Bruno Schüty, Christoph Gohlke, and Leonel Malacrida.
PhasorPy: un nuevo software abierto para el análisis de imágenes espectrales y tiempo de vida por gráficos de fasores.
<a class="reference external" href="https://www.semanacademica.hc.edu.uy/index.php/galeria2024/681">Semana Académica del Hospital de Clínicas</a>.
September 23-27, 2024. Montevideo, Uruguay.</p></li>
<li><p>Leonel Malacrida.
Phasor analysis for FLIM & STED.
<a class="reference external" href="https://www.canadabioimaging.org/mlmc-infocus-sted">Montreal Light Microscopy Course (MLMC). In-Focus: STimulated Emission Depletion (STED) Microscopy</a>.
July 22-26, 2024. Montreal, Canada.</p></li>
<li><p>Leonel Malacrida.
The phasor approach for biophysical studies of condensates and membranes using DAN probes.
<a class="reference external" href="https://www.sibpa.it/CongressoNazionaleSIBPAGenova/">XXVII Congreso Nazionale SIBPA</a>.
June 16-20, 2024. Genoa, Italy.</p></li>
<li><p>Leonel Malacrida.
PhasorPy: a Python open-source library for phasor analysis of FLIM and HSI data.
<a class="reference external" href="https://chanzuckerberg.com/science/programs-resources/open-science/">CZI Open Science 2024</a>.
June 11-14, 2024. Boston, USA.</p></li>
<li><p>Leonel Malacrida.
The phasor plots for time-resolved and hyperspectral imaging: fundamentals and new developments.
<a class="reference external" href="https://www.samictucuman2024.com/">Sociedad Argentina de Microscopia</a>.
May 29-31, 2024. Tucuman, Argentina.</p></li>
<li><p>Leonel Malacrida.
The phasor plot analysis for hyperspectral imaging and time-resolved imaging: PhasorPy developments and its applications.
<a class="reference external" href="https://librehub.github.io/2024/04/03/leonel-malacrida.html">Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub) Seminars</a>.
May 15, 2024. Online.
<a class="reference external" href="https://www.youtube.com/watch?v=CbmDNjwo_sg">Recording</a>.</p></li>
<li><p>Leonel Malacrida.
PhasorPy: a Python open-source library as SimFCS legacy for phasor analysis of FLIM and HSI data.
<a class="reference external" href="https://www.lfd.uci.edu/frontiers/">Frontiers in Biological Fluorescence 2024</a>.
May 10, 2024. University of California, Irvine, USA.</p></li>
<li><p>Leonel Malacrida.
Microscopía no lineal en Uruguay: 2P-FLIM y microscopio DIVER para estudiar procesos in vivo en la profundidad del tejido.
<a class="reference external" href="https://cicada.uy/simposio-en-microscopia-y-bioimagenes-avances-y-desafios/">Simposio en Microscopía y Bioimágenes: avances y desafíos</a>.
April 4-5, 2024. University of the Republic (UdelaR). Montevideo, Uruguay.</p></li>
<li><p>Leonel Malacrida.
Microscopy techniques applied to the analysis of biosensors performance.
<a class="reference external" href="https://pasteur.uy/cursos-y-charlas/1st-latin-american-workshop-development-and-applications-of-biosensors-from-fluorescent-proteins-to-synthetic-biology/">1st Latin-American Workshop: Development and applications of biosensors: from fluorescent proteins to synthetic biology</a>.
Nov 27 - Dec 1, 2023. Institut Pasteur de Montevideo, Uruguay.</p></li>
<li><p>Bruno Schüty, Bruno Pannunzio, and Leonel Malacrida.
Practical section 3: PhasorPy and Napari plugin for FLIM & HSI analysis.
<a class="reference external" href="https://pasteur.uy/2023/5th-workshop-in-advanced-microscopy-and-biophotonics/">5th Workshop in Advanced Microscopy and Biophotonics</a>.
November 19-24, 2023. Institut Pasteur de Montevideo, Uruguay.</p></li>
<li><p>Christoph Gohlke and Leonel Malacrida.
Introduction to PhasorPy.
<a class="reference external" href="https://www.lfd.uci.edu/workshop/">17th LFD Workshop in Advanced Fluorescence Imaging and Dynamics</a>.
October 23-27, 2023. Laboratory for Fluorescence Dynamics,
University of California, Irvine, USA.</p></li>
</ul>
</section>
<section id="publications">
<h2>Publications<a class="headerlink" href="#publications" title="Link to this heading">#</a></h2>
<p>Publications using PhasorPy:</p>
<ol class="arabic simple">
<li><p>González-Gutiérrez M, Vázquez-Enciso DM, Mateos N, Hwang W, Torres-García E, Hernández HO, Chacko JV, Coto Hernández I, et al.
<a class="reference external" href="https://doi.org/10.64898/2026.03.17.712453">PSF-driven spatio-temporal blending in fluorescence lifetime imaging microscopy and its mitigation via mean-shift super-resolution-based masking</a>.
<em>bioRxiv</em> (2026)</p></li>
<li><p>Schuty B, Garcia MJ, Khuon S, Malacrida L.
<a class="reference external" href="https://doi.org/10.1016/j.sbsr.2026.101014">Phasor analysis of RGB camera data enables fluorescence microscopy unmixing and brightfield segmentation in a commercial microscope</a>.
<em>Sens Bio-Sens Res</em>. 52: 101014 (2026)</p></li>
<li><p>Prieto D, Rehermann MI, Fabbiani G, Vitar M, Trujillo-Cenoz O, Falco MV, Cuparo M, Trigo FF, et al.
<a class="reference external" href="https://doi.org/10.64898/2026.04.09.713694">A filopodia-based dendritic mechanosensory compartment in CSF-contacting neurons</a>.
<em>bioRxiv</em> (2026)</p></li>
<li><p>Halbers LP, Brennan CK, Scipioni L, Tedeschi G, Torrey ZR, Parag-Sharma K, Labra B, Gohlke C, et al.
<a class="reference external" href="https://doi.org/10.1016/j.crmeth.2026.101344">Expanded applications of bioluminescence microscopy with phasor analysis</a>.
<em>Cell Rep Methods</em>. 6(4): 101344 (2026)</p></li>
<li><p>Fuller EB, Cole KH, Halbers LP, Chan CET, Ng KK, Scipioni L, Gohlke C, Digman MA, et al.
<a class="reference external" href="https://doi.org/10.1021/jacs.5c16597">Bioluminescent probes for multiplexed RNA imaging</a>.
<em>J Am Chem Soc</em>. 148(11): 11521-11530 (2026)</p></li>
<li><p>Sabri E, Mangiarotti A, Dimova R.
<a class="reference external" href="https://doi.org/10.1038/s41467-026-71273-7">Fluorescence-based mapping of condensate dielectric permittivity uncovers hydrophobicity-driven membrane interactions</a>.
<em>Nat Commun</em>. 17:3155 (2026)</p></li>
<li><p>Pannunzio B, Cespedes P, Diaz M, Ali D, Rial A, Malacrida L.
<a class="reference external" href="https://doi.org/10.64898/2026.02.27.708623">High-throughput single-cell spectroscopy using phasor analysis of spectral flow cytometry</a>.
<em>bioRxiv</em> (2026)</p></li>
<li><p>Zhao W, Samimi K, Skala MC, Datta R.
<a class="reference external" href="https://doi.org/10.1101/2025.09.30.679625">FLIM playground: an interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy</a>.
<em>bioRxiv</em> (2025)</p></li>
<li><p>Sabri E, Mangiarotti A, Schmitt CNZ, Dimova R.
<a class="reference external" href="https://doi.org/10.1101/2025.10.20.683555">Seeing the chemistry of biomolecular condensates: in situ mapping of composition and water content</a>.
<em>bioRxiv</em> (2025)</p></li>
</ol>
<p>Publications mentioning PhasorPy:</p>
<ol class="arabic simple">
<li><p>Harkhoe R, Ferrari G, Dmitriev RI, Kuhl M.
<a class="reference external" href="https://doi.org/10.1021/acsmeasuresciau.5c00209">Luminescence lifetime imaging: Key concepts and advances in the image acquisition, processing, and analysis pipeline</a>.
<em>ACS Meas Sci Au</em>. acsmeasuresciau.5c00209 (2026)</p></li>
<li><p>Georgakoudi I, Skala MC, Quinn KP, Stringari C, Sorrells JE, Heikal AA, Li LZ, Xu HN, et al.
<a class="reference external" href="https://doi.org/10.1117/1.JBO.30.S2.S23901">Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling</a>.
<em>J Biomed Opt</em>. 30(S2): S23901 (2025)</p></li>
<li><p>Wetzker C, Zoccoler ML, Iarovenko S, Okafornta CW, Nobst A, Hartmann H, Muller-Reichert T, Haase R, et al.
<a class="reference external" href="https://doi.org/10.1111/jmi.70036">A fluorescence lifetime separation approach for FLIM live-cell imaging</a>.
<em>J Microscopy</em>. 301(1): 91-106 (2025)</p></li>
<li><p>Wetzker C, Zoccoler ML, Iarovenko S, Okafornta CW, Nobst A, Hartmann H, Müller-Reichert T, Haase R, et al.
<a class="reference external" href="https://doi.org/10.1101/2025.03.05.641717">Fluorescence lifetime unmixing: A new workflow for FLIM live-cell imaging</a>.
<em>bioRxiv</em> (2025)</p></li>
<li><p>Michalet X.
<a class="reference external" href="https://doi.org/10.1101/2025.05.22.655640">AlliGator: open source fluorescence lifetime imaging analysis in G</a>.
<em>bioRxiv</em> (2025)</p></li>
<li><p>Kapsiani S, Laubli NF, Ward EN, Shehata M, Kaminski CF, Kaminski Schierle GS.
<a class="reference external" href="https://doi.org/10.1021/acs.analchem.5c00495">FLIMPA: a versatile software for fluorescence lifetime imaging microscopy phasor analysis</a>.
<em>Anal Chem</em>. 97(22): 11382-11387 (2025)</p></li>
<li><p>Vallmitjana A, Torrado B, Durkin AF, Dvornikov A, Rajil N, Ranjit S, Balu M.
<a class="reference external" href="https://doi.org/10.1093/bioinformatics/btaf162">GSLab: open-source platform for advanced phasor analysis in fluorescence microscopy</a>.
<em>Bioinformatics</em>. 41(4): btaf162 (2025)</p></li>
<li><p>Weber F, Iskrak S, Ragaller F, Schlegel J, Plochberger B, Sezgin E,
Andronico LA.
<a class="reference external" href="https://doi.org/10.1242/jcs.262166">VISION - an open-source software for automated multi-dimensional image analysis of cellular biophysics</a>.
<em>J Cell Sci</em>. 137(20): jcs262166 (2024)</p></li>
<li><p>Mangiarotti A, Dimova R.
<a class="reference external" href="https://doi.org/10.1016/bs.mie.2024.01.024">Chapter five - The spectral phasor approach to resolving membrane order with environmentally sensitive dyes</a>.
<em>Methods Enzymol</em>. 700:105-126 (2024)</p></li>
</ol>
</section>
<section id="cite">
<h2>Cite<a class="headerlink" href="#cite" title="Link to this heading">#</a></h2>
<p>Please cite
<a class="reference external" href="https://dx.doi.org/10.5281/zenodo.13862586">doi: 10.5281/zenodo.13862586</a>
if PhasorPy contributes to research that leads to a publication.</p>
</section>
<section id="contact">
<h2>Contact<a class="headerlink" href="#contact" title="Link to this heading">#</a></h2>
<p>PhasorPy is a community-maintained project.</p>
<p><a class="reference external" href="docs/stable/contributing/">Contributions</a>
in the form of bug reports, bug fixes, feature implementations, documentation,
datasets, and enhancement proposals are welcome.</p>
<p>Report issues and ask questions about PhasorPy on the GitHub
<a class="reference external" href="https://github.com/phasorpy/phasorpy/issues">issue tracker</a>.</p>
<p>Alternatively, contact the
<a class="reference external" href="https://github.com/orgs/phasorpy/people">PhasorPy developers</a> directly.</p>
</section>
</section>
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